Anti-PIM1 (phospho Y309) 抗体 (ab74047)
Key features and details
- Rabbit polyclonal to PIM1 (phospho Y309)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PIM1 (phospho Y309) antibody
PIM1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PIM1 (phospho Y309) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human PIM1 (phospho Y309).
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ポジティブ・コントロール
- HUVEC cell extracts treated with PMA (125ng/ml, 30mins).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Frozen Stock (-20C). Shelf life 12 months. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab74047 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74047の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). |
ターゲット情報
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機能
May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis. -
組織特異性
Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated on both serine/threonine and tyrosine residues. -
細胞内局在
Cytoplasm. Nucleus and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5292 Human
- Entrez Gene: 18712 Mouse
- Entrez Gene: 24649 Rat
- Omim: 164960 Human
- SwissProt: P11309 Human
- SwissProt: P06803 Mouse
- SwissProt: P26794 Rat
- Unigene: 81170 Human
see all -
別名
- Oncogene PIM 1 antibody
- Oncogene PIM1 antibody
- PIM 1 antibody
see all
画像
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All lanes : Anti-PIM1 (phospho Y309) antibody (ab74047) at 1/500 dilution
Lane 1 : HUVEC cell extracts
treated with PMA (125ng/ml, 30mins)
Lane 2 : HUVEC cell extracts
treated with PMA (125ng/ml, 30mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab74047 は論文での使用が確認できていません。