Anti-PIM1 抗体 [ZP003] (ab54503)

製品の概要

  • 製品名Anti-PIM1 antibody [ZP003]
    PIM1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [ZP003] to PIM1
  • 特異性ab54503 is specific for the PIM1 protein.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from the internal region of the human PIM1 protein, which is 94% and 88% homologous with rat and mouse, respectively.

  • ポジティブ・コントロール
    • WB: K562 cytoplasmic or nuclear lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa.
ELISA Use a concentration of 0.1 - 1 µg/ml.

ターゲット情報

  • 機能May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.
  • 組織特異性Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated on both serine/threonine and tyrosine residues.
  • 細胞内局在Cytoplasm. Nucleus and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Oncogene PIM 1 antibody
    • Oncogene PIM1 antibody
    • PIM 1 antibody
    • pim 1 kinase 44 kDa isoform antibody
    • Pim 1 kinase antibody
    • pim 1 oncogene (proviral integration site 1) antibody
    • Pim 1 oncogene antibody
    • PIM antibody
    • PIM1 antibody
    • pim1 kinase 44 kDa isoform antibody
    • PIM1_HUMAN antibody
    • Pim2 antibody
    • PIM3 antibody
    • Proto oncogene serine/threonine protein kinase Pim 1 antibody
    • Proto-oncogene serine/threonine-protein kinase Pim-1 antibody
    • Proviral integration site 1 antibody
    • Proviral integration site 2 antibody
    see all

Anti-PIM1 antibody [ZP003] 画像

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PIM1 knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab54503 observed at 80 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab54503 was shown to recognize PIM1 when PIM1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PIM1 knockout samples were subjected to SDS-PAGE. ab54503 and ab181602 (loading control to GAPDH) were  diluted 3 μg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Anti-PIM1 antibody [ZP003] (ab54503) at 3 µg/ml + K562 cell lysate

    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Anti-PIM1 antibody [ZP003] (ab54503) 使用論文

ab54503 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Here are my answers to your questions. Is there a loading limit (cell number) for sample buffer as the lysis buffer? We recommend 1 ml of lysis buffer per 10 million cells. For sample buffer, if you mean gel loading b...

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Thank you for contacting us with your question. It will not make a difference if the host of your secondary antibodies is the same or different, however if you will be using two primaries from different species on the same tissue, it will be bes...

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