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A synthetic peptide corresponding to residues in human PIM1
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab75776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/10000 - 1/20000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). Also detects a band of approximately 36kDa (isoform 2). The 45 kDa band is isoform 1.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Blocking buffer and concentration
Diluting buffer and concentration
5% NFDM /TBST
Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling PIM1 with purified ab75776 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised using 90% methanol. A goat anti rabbit IgG (Alexa Fluor®488) (ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody