製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PIM1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MLLSKINSLA HLRAAPCNDL HATKLAPGKE KEPLESQYQV GPLLGSGGFG) of Human PIM1 (NP_002639).

  • ポジティブ・コントロール
    • Fetal heart lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab98004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.
  • 組織特異性
    Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
  • 配列類似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Autophosphorylated on both serine/threonine and tyrosine residues.
  • 細胞内局在
    Cytoplasm. Nucleus and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Oncogene PIM 1 antibody
    • Oncogene PIM1 antibody
    • PIM 1 antibody
    • pim 1 kinase 44 kDa isoform antibody
    • Pim 1 kinase antibody
    • pim 1 oncogene (proviral integration site 1) antibody
    • Pim 1 oncogene antibody
    • PIM antibody
    • PIM1 antibody
    • pim1 kinase 44 kDa isoform antibody
    • PIM1_HUMAN antibody
    • Pim2 antibody
    • PIM3 antibody
    • Proto oncogene serine/threonine protein kinase Pim 1 antibody
    • Proto-oncogene serine/threonine-protein kinase Pim-1 antibody
    • Proviral integration site 1 antibody
    • Proviral integration site 2 antibody
    see all

画像

  • Anti-PIM1 antibody (ab98004) at 1 µg/ml (5% skim milk/ PBS buffer) + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa

プロトコール

参考文献

ab98004 has not yet been referenced specifically in any publications.

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