製品の概要

  • 製品名Anti-PIGY antibody
    PIGY 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PIGY
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    amino acids 23-114 (VARRCLHASG SRPLADRGKK TEEPPRDFDP ALLEFLVCPL SKKPLRYEAS TNELINEELG IAYPIIDGIP NMIPQAARMT RQSKKQEEVE QR) of Human PIGY.

  • ポジティブ・コントロール
    • Human adrenal gland tissue; RT-4, U-251 MG, Human liver and tonsil lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

ターゲット情報

  • 関連性This gene encodes two proteins, one of which is part of the GPI-N-acetylglucosaminyltransferase (GIP-GnT) complex which initiates the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is synthesized in the endoplasmic reticulum and serves as an anchor for many surface proteins. Proteins containing GPI anchors can have an important role in cell-cell interactions. Two open reading frames have been found in the single transcript that has been identified for this gene. The downstream open reading frame encodes the GPI-GnT complex protein while the upstream open reading frame encodes a protein with unknown function.
  • 細胞内局在Endoplasmic reticulum membrane. Plasma membrane.
  • 参照データベース
  • 別名
    • Phosphatidylinositol glycan anchor biosynthesis, class Y antibody
    • Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y antibody
    • Phosphatidylinositol-glycan biosynthesis class Y protein antibody
    • PIG Y antibody
    • PIG-Y antibody
    see all

Anti-PIGY antibody 画像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab122338 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122338, at a 1/75 dilution, staining PIGY in paraffin-embedded Human adrenal gland tissue by immunohistochemistry.
  • All lanes : Anti-PIGY antibody (ab122338) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

Anti-PIGY antibody (ab122338) 使用論文

ab122338 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"