製品の概要

  • 製品名Anti-PIGT antibody
    PIGT 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PIGT
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 432-481 (IQLPANSVTK VSIQFERALL KWTEYTPDPN HGFYVSPSVL SALVPSMVAA) of Human PIGT (NP_057021).

  • ポジティブ・コントロール
    • Transfected 293T cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

ターゲット情報

  • 関連性PIGT is an essential component of the multisubunit enzyme, GPI transamidase and is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells which serves to anchor proteins to the cell surface. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.
  • 細胞内局在Endoplasmic reticulum membrane; Single-pass type I membrane protein.
  • 参照データベース
  • 別名
    • CGI 06 antibody
    • FLJ41596 antibody
    • GPI transamidase component PIG T antibody
    • GPI transamidase subunit antibody
    • MGC8909 antibody
    • NDAP antibody
    • Neurotrophin regulated neuronal development associated protein antibody
    • Phosphatidyl inositol glycan class T antibody
    • Phosphatidylinositol glycan anchor biosynthesis class T antibody
    • Phosphatidylinositol glycan class T antibody
    see all

Anti-PIGT antibody 画像

  • Anti-PIGT antibody (ab80888) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)

Anti-PIGT antibody (ab80888) 使用論文

ab80888 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"