製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PIGR
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 180-429 of Human PIGR.

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLa, HepG2, Molt4 and Raji cells; Cal27 xenograft tissue.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/300. Predicted molecular weight: 83 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.
  • 配列類似性
    Contains 5 Ig-like V-type (immunoglobulin-like) domains.
  • 翻訳後修飾
    N-glycosylation is not necessary for Ig binding.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Hepatocellular carcinoma associated protein TB6 antibody
    • Hepatocellular carcinoma-associated protein TB6 antibody
    • MGC125361 antibody
    • MGC125362 antibody
    • Phosphatidylinositol glycan, class R antibody
    • PIGR antibody
    • PIGR_HUMAN antibody
    • Poly Ig receptor antibody
    • Poly-Ig receptor antibody
    • Polymeric immunoglobulin receptor antibody
    • Secretory component antibody
    • Transmembrane secretory component antibody
    see all

画像

  • Anti-PIGR antibody (ab96196) at 1/1000 dilution + Molt4 whole cell lysate at 30 µg

    Predicted band size : 83 kDa
  • ab96196 at 1/200 dilution staining PIGR in methanol fixed HepG2 cells. Lower image is co-stained with Hoechst 33342.
  • ab96196 at 1/500 dilution staining PIGR in paraffin-embedded Cal27 xenograft tissue.

参考文献

ab96196 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat Hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat Hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Dec 21 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (6% SDS-PAGE)
Loading amount
15 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Nov 17 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (WIF-B cells, the hepatoma-derived hybrid cell line)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
35 µg
Specification
WIF-B cells, the hepatoma-derived hybrid cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Nov 04 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (WIF-B cells, the hepatoma-derived hybrid cell line)
Permeabilization
Yes - 0.2% Triton X-100
Specification
WIF-B cells, the hepatoma-derived hybrid cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Oct 17 2016

DISCOUNT CODES:

For Ab17377: ***
For Ab96196: ***

Expiration date: February 18, 2013

Value: $337 each

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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