The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human PiGF.
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to PIGF (Biotin) (ab84226). Using ab84226 as the detection antibody and ab110624 as the capture antibody, the detection limit for recombinant Human PiGF is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 25 kDa. The detection limit for recombinant Human PiGF is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
Involved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI.
Mohammed KA et al. Cyclic stretch induces PlGF expression in bronchial airway epithelial cells via nitric oxide release. Am J Physiol Lung Cell Mol Physiol292:L559-66 (2007).
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