製品の概要

  • 製品名
    Anti-PiGF antibody
    PiGF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PiGF
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: Indirect ELISA, Sandwich ELISA, WBmore details
  • 種交差性
    交差種: Recombinant fragment
    交差が予測される動物種: Human
  • 免疫原

    Highly pure (>98%) recombinant Human PiGF

製品の特性

  • 製品の状態
    Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab110624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human PiGF.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to PIGF (Biotin) (ab84226). Using ab84226 as the detection antibody and ab110624 as the capture antibody, the detection limit for recombinant Human PiGF is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 25 kDa. The detection limit for recombinant Human PiGF is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

ターゲット情報

  • 機能
    Involved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI.
  • パスウェイ
    Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
  • 配列類似性
    Belongs to the PIGF family.
  • 細胞内局在
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • GPI11 homolog antibody
      • MGC32646 antibody
      • MGC33136 antibody
      • PHOSPHATIDYLINOSITOL GLYCAN antibody
      • Phosphatidylinositol glycan biosynthesis class F protein antibody
      • Phosphatidylinositol-glycan biosynthesis class F protein antibody
      • PIG F antibody
      • PIG-F antibody
      • PIGF antibody
      • PIGF_HUMAN antibody
      • PIGF1 antibody
      see all

    プロトコール

    参考文献

    This product has been referenced in:
    • Mohammed KA  et al. Cyclic stretch induces PlGF expression in bronchial airway epithelial cells via nitric oxide release. Am J Physiol Lung Cell Mol Physiol 292:L559-66 (2007). Read more (PubMed: 17028267) »

    See 1 Publication for this product

    ab110624 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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