製品の概要
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製品名Anti-PiGF antibody
PiGF 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to PiGF
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アプリケーション適用あり: Indirect ELISA, Sandwich ELISA, WBmore details
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種交差性交差種: Recombinant fragment
交差が予測される動物種: Human
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免疫原
Highly pure (>98%) recombinant Human PiGF
製品の特性
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製品の状態Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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バッファーpH: 7.20
Constituent: PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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sELISA pair antibody
アプリケーション
Our Abpromise guarantee covers the use of ab110624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Indirect ELISA | Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human PiGF. | |
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to PIGF (Biotin) (ab84226). Using ab84226 as the detection antibody and ab110624 as the capture antibody, the detection limit for recombinant Human PiGF is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution. | |
| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 25 kDa. The detection limit for recombinant Human PiGF is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions. |
ターゲット情報
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機能Involved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI.
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パスウェイGlycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
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配列類似性Belongs to the PIGF family.
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細胞内局在Endoplasmic reticulum membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 5281 Human
- Omim: 600153 Human
- SwissProt: Q07326 Human
- Unigene: 468415 Human
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別名
- GPI11 homolog antibody
- MGC32646 antibody
- MGC33136 antibody
see all
プロトコール
Anti-PiGF antibody (ab110624) 使用論文
This product has been referenced in:
- Mohammed KA et al. Cyclic stretch induces PlGF expression in bronchial airway epithelial cells via nitric oxide release. Am J Physiol Lung Cell Mol Physiol 292:L559-66 (2007). Read more (PubMed: 17028267) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"