Pig TNF alpha ELISA Kit (ab100756)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 0.049 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
製品の概要
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製品名
Pig TNF alpha ELISA Kit
TNF alpha キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 20 pg/ml -
検出範囲
0.049 ng/ml - 10 ng/ml -
添加回収試験
100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 132.1 123% - 142% Serum 111.7 102% - 121% Plasma 97.02 70% - 126% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Pig -
製品の概要
Abcam’s TNF alpha Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Pig TNF-alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Pig TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Pig TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Pig TNF alpha 2 vials Recombinant Pig TNF alpha Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml TNF alpha Microplate (12 x 8 wells) 1 unit -
研究分野
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機能
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
関連疾患
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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別名
- APC1
- APC1 protein
- Cachectin
see all -
参照データベース
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab100756 は 9 報の論文で使用されています。
- Jia D et al. Ischemic postconditioning improves the outcome of organs from donors after cardiac death in a pig liver transplantation model and provides synergistic protection with hypothermic machine perfusion. Clin Transplant 35:e14417 (2021). PubMed: 34231926
- Wan K et al. Effects of dietary calcium pyruvate on gastrointestinal tract development, intestinal health and growth performance of newly weaned piglets fed low-protein diets. J Appl Microbiol 128:355-365 (2020). PubMed: 31618501
- Zhang S et al. Immune response in piglets orally immunized with recombinant Bacillus subtilis expressing the capsid protein of porcine circovirus type 2. Cell Commun Signal 18:23 (2020). PubMed: 32046726
- Zhang ZB et al. Normothermic Machine Perfusion Protects Against Liver Ischemia-Reperfusion Injury During Reduced-Size Liver Transplantation in Pigs. Ann Transplant 24:9-17 (2019). PubMed: 30607000
- Adams S et al. Effects of pulverized oyster mushroom (Pleurotus ostreatus) on diarrhea incidence, growth performance, immunity, and microbial composition in piglets. J Sci Food Agric 99:3616-3627 (2019). PubMed: 30628086
- Huang HN et al. Epinecidin-1 Protects against Methicillin Resistant Staphylococcus aureus Infection and Sepsis in Pyemia Pigs. Mar Drugs 17:N/A (2019). PubMed: 31835381
- Zhang N et al. Dapagliflozin improves left ventricular remodeling and aorta sympathetic tone in a pig model of heart failure with preserved ejection fraction. Cardiovasc Diabetol 18:107 (2019). PubMed: 31429767
- Xu M et al. Anti-CD47 monoclonal antibody therapy reduces ischemia-reperfusion injury of renal allografts in a porcine model of donation after cardiac death. Am J Transplant 18:855-867 (2018). PubMed: 29087049
- Yeung CC et al. An ex vivo porcine skin model to evaluate pressure-reducing devices of different mechanical properties used for pressure ulcer prevention. Wound Repair Regen 24:1089-1096 (2016). PubMed: 27717144