製品の概要

  • 製品名Pig IL-1 beta ELISA Kit
    IL1 beta キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 6 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    90 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 96.91 89% - 104%
    Serum 97.32 88% - 105%
    Plasma 87.75 80% - 92%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Pig
  • 製品の概要

    Abcam's IL1 beta Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of pig IL1 beta in serum, plasma and cell culture supernatants.


     


    This assay employs an antibody specific for IL1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pig IL1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    For higher sensitivity and reproducibility in only 90 minutes, we recommend Mouse IL-1 beta ELISA Kit (ab197742) from our SimpleStep ELISA® range.


     

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 配列類似性Belongs to the IL-1 family.
  • ドメインThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • 細胞内局在Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • 別名
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab100754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Pig IL-1 beta ELISA Kit 画像

  • Representative Standard Curve using ab100754

プロトコール

Pig IL-1 beta ELISA Kit (ab100754) 使用論文

ab100754 has not yet been referenced specifically in any publications.

Product Wall

Application ELISA
Sample Pig Serum (cell extracts)
Type of Kit Used ELISA
Specification cell extracts
Did you use the Abcam Kit protocol? Yes
Amount of Sample 100 µg
Positive control standard provided by kit
Username

Dr. Carolina Velez

Verified customer

投稿 Feb 22 2016

Abreview for IL1 beta Pig ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Pig Serum (Blood)
Specification: Blood
Amount of Sample: 100 µg
Type of Kit Used: ELISA
Positive control: Standards IL1 Beta
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Serum samples were analyzed undiluted, all samples were within the detection range of the kit, and the calibration curve was well adjusted (R2: 0.9992).
Username

Abcam user community

Verified customer

投稿 Mar 26 2013

Thank you for contacting us.

I have received following reply from my colleague in laboratory;

I’m not exactly sure what the customer means by “one recovery at a high concentration” as the Spiking and Recovery QC data...

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Thank you for contacting us.

This kit is developed by one of our collaborator. Our collaborator product as this one has passed strict quality checks we have at Abcam which is why we are selling this kit. The product has been fully tested with ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"