製品の概要

  • 製品名PicoProbe Acetyl CoA Assay Kit
  • テスト
    100 x 1 assay
  • サンプルの種類
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • アッセイタイプQuantitative
  • 検出感度
    > 0.01 nmol/well
  • 検出範囲
    0.01 nmol/well - 1 nmol/well
  • 製品の概要

    Abcam's PicoProbe Acetyl CoA Assay Kit is a highly sensitive assay for determining Acetyl CoA level in a variety of biological samples. In the assay, free CoA is quenched then Acetyl CoA is converted to CoA. The CoA is reacted to form NADH which interacts with PicoProbe to generate fluorescence (Ex=535/Em=587 nm). The assay can detect 10 to 1000 pmol of Acetyl CoA (with detection limit ~0.4 µM) in a variety of samples.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Acetyl CoA is a central molecule of metabolism. It carries acetate, used in the build-up and breakdown of larger molecules. Acetyl CoA is key in synthetic pathways leading to sesquiterpenes, precursors to cholesterol and other sterols, flavenoids and other polyketides, polyenes and long-chain fatty acids. It is the source of the acetyl group used in histone acetylation. The acetyl group is also incorporated into a variety of other molecules such as acetylcholine, melatonin, heme and TCA cycle intermediates.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Acetyl CoA (AcCoA) is the metabolic intermediate which transfers carbon atoms to the TCA cycle for energy production and is the link between fat and carbohydrate metabolism. When fatty acid levels are high, AcCoA levels rise beyond the energy requirements of the organism and the excess is converted to the ketone bodies acetone and ß-hydroxybutyrate. AcCoA also contributes acetate to histone acetylation and isoprenoid synthesis.
  • 別名
    • AcCoA

アプリケーション

Our Abpromise guarantee covers the use of ab87546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

PicoProbe Acetyl CoA Assay Kit 画像

  • Examples of standard curves generated following the ab87546 kit protocol.

プロトコール

PicoProbe Acetyl CoA Assay Kit (ab87546) 使用論文

This product has been referenced in:
  • Zhang Y  et al. Induction of Posttranslational Modifications of Mitochondrial Proteins by ATP Contributes to Negative Regulation of Mitochondrial Function. PLoS One 11:e0150454 (2016). Functional Studies . Read more (PubMed: 26930489) »
  • Teng CF  et al. Activation of ATP citrate lyase by mTOR signal induces disturbed lipid metabolism in hepatitis B virus pre-S2 mutant tumorigenesis. J Virol 89:605-14 (2015). Read more (PubMed: 25339766) »

See all 8 Publications for this product

Product Wall



J’ai contacté le laboratoire concernant le kit ab87546 :

1.Nous recommandons d’utiliser ˜2-5 X 10^6 cellules pour chaque manip.

2. Il est recommandé de faire une déprotéinisation pour des cellules en culture. J’ai attaché ...

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I discussed this with the laboratories. They confirmed that the sample can be stored at -80 degree centigrade once prepared. However, we recommend to use the sample immediately after preparation for maximum efficiency.

I discussed this with the laboratories. They confirmed that the sample can be stored at -80 degree centigrade once prepared. However, we recommend to use the sample immediately after preparation for maximum efficiency.


In principle, eliminating the quenching and conversion step will allow you to measure CoA alone. However, the kit was not produced nor validated for this purpose, so we cannot guarantee that it will work, nor do we have a CoA standard that has been val...

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I am happy to confirm that kit PicoProbe Acetyl CoA Assay Kit (ab87546) can be used with frozen mouse tissues.

Please find a protocol for tissue preparation below:

1. Tissue samples: (20 - 1000 mg) should be frozen rapidly (liqui...

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The ab138889 CoA detection kit assay buffer is 100 mM Na phosphate buffer, pH = 6. The RIPA buffer may be an issue with the other two kits, since they are enzyme-based, and that kind of assay is generally incompatible with detergents. Your best approac...

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1. Mitochonrial lysate can be assayed by ab87546.

2. For the amount sample to be added into each sample well, customer is recommended to perform a pilot experiment with a range of dilutions of the sample to determine the optimal concentratio...

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It is not the best case scenario to freeze samples repeatedly before performing the assay. However, if you need to freeze the samples, it would be possible after separating the sample from the KClO4 pellet.


I have contacted the lab and unfortunately, this is only a fluorometric assay kit and cannot be used colorimetrically.

Thank you for contacting us.

This kit is actually based on reaction of breakdown of Acety-CoA into CoA and acetyl group. This is inverse to what you are looking for. The kit have enzyme which actualy breakdown acetyl CoA rather than synthesizi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"