製品の概要

  • 製品名Anti-PICK1 antibody
    PICK1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to PICK1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Horse, Chicken, Cow, Dog, Human, Pig, Xenopus laevis, Chimpanzee
  • 免疫原

    Synthetic peptide:

    C-HYNKLQADPKQ

    , corresponding to internal sequence amino acids 100-110 of Human PICK1 (NP_036539.1; NP_001034672.1; NP_001034673.1)

  • ポジティブ・コントロール
    • Mouse and Rat brain lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 47 kDa.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 機能Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel.
  • 組織特異性Ubiquitous.
  • 配列類似性Contains 1 AH domain.
    Contains 1 PDZ (DHR) domain.
  • 翻訳後修飾Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors.
  • 細胞内局在Cytoplasm > perinuclear region. Cell junction > synapse. Also present at excitatory synapses.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ1039K5 antibody
    • MGC15204 antibody
    • OTTHUMP00000028509 antibody
    • PICK 1 antibody
    • Pick1 antibody
    • PICK1_HUMAN antibody
    • PRKCA binding protein antibody
    • PRKCA-binding protein antibody
    • PRKCABP antibody
    • Protein interacting with C kinase 1 antibody
    • Protein interacting with PRKCA antibody
    • Protein interacting with PRKCA 1 antibody
    • Protein kinase C alpha binding protein antibody
    • Protein kinase C-alpha-binding protein antibody
    • Protein that interacts with C kinase 1 antibody
    see all

Anti-PICK1 antibody 画像

  • Anti-PICK1 antibody (ab77178) at 1 µg/ml + mouse brain lysate in RIPA buffer at 35 µg

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab77178 staining PICK1 in CHO cells stably transfected with Human PICK1 by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 1 hour at room temperature. The primary antibody was diluted 1/1000 in PBS, 0.5% Triton, 10% FCS and incubated with the sample for 2 hours at °C. The secondary antibody was Alexa Fluor® 488-conjugated Donkey anti-Goat polyclonal, diluted 1/500.

    See Abreview

Anti-PICK1 antibody (ab77178) 使用論文

ab77178 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Chinese Hamster Cultured Cells (CHO Cells stably transfected with human Pick1)
Specification CHO Cells stably transfected with human Pick1
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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投稿 May 03 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Stably transfected CHO cells expressing human Pick)
Loading amount 20 µg
Specification Stably transfected CHO cells expressing human Pick
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Feb 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"