製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to PICK1
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Horse, Chicken, Cow, Dog, Human, Pig, Xenopus laevis, Chimpanzee
  • 免疫原

    Synthetic peptide:

    C-HYNKLQADPKQ

    , corresponding to internal sequence amino acids 100-110 of Human PICK1 (NP_036539.1; NP_001034672.1; NP_001034673.1)

  • ポジティブ・コントロール
    • Mouse and Rat brain lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 47 kDa.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Contains 1 AH domain.
    Contains 1 PDZ (DHR) domain.
  • 翻訳後修飾
    Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors.
  • 細胞内局在
    Cytoplasm > perinuclear region. Cell junction > synapse. Also present at excitatory synapses.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ1039K5 antibody
    • MGC15204 antibody
    • OTTHUMP00000028509 antibody
    • PICK 1 antibody
    • Pick1 antibody
    • PICK1_HUMAN antibody
    • PRKCA binding protein antibody
    • PRKCA-binding protein antibody
    • PRKCABP antibody
    • Protein interacting with C kinase 1 antibody
    • Protein interacting with PRKCA antibody
    • Protein interacting with PRKCA 1 antibody
    • Protein kinase C alpha binding protein antibody
    • Protein kinase C-alpha-binding protein antibody
    • Protein that interacts with C kinase 1 antibody
    see all

画像

  • Anti-PICK1 antibody (ab77178) at 1 µg/ml + mouse brain lysate in RIPA buffer at 35 µg

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab77178 staining PICK1 in CHO cells stably transfected with Human PICK1 by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 1 hour at room temperature. The primary antibody was diluted 1/1000 in PBS, 0.5% Triton, 10% FCS and incubated with the sample for 2 hours at °C. The secondary antibody was Alexa Fluor® 488-conjugated Donkey anti-Goat polyclonal, diluted 1/500.

    See Abreview

参考文献

ab77178 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Chinese Hamster Cultured Cells (CHO Cells stably transfected with human Pick1)
Specification
CHO Cells stably transfected with human Pick1
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Verified customer

投稿 May 03 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Stably transfected CHO cells expressing human Pick)
Loading amount
20 µg
Specification
Stably transfected CHO cells expressing human Pick
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

投稿 Feb 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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