Rabbit polyclonal to Piccolo
No cross-reactivity to Bassoon. Multiple isoforms can be detected.
Mouse, Rat, Human
Full length Human Piccolo protein (BC001304.1).
Mouse and Rat brain lysate; Nuromuscular junction muscle samples from adult mouse.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Constituent: PBS Note: 50% Glycerol
0.09% Sodium Azide
Concentration information loading...
Protein G purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 567 kDa. For large isoforms reduce dilution.
May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking.
Contains 2 C2 domains.
Contains 1 PDZ (DHR) domain.
C2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change.
Cell junction > synapse. Concentrated at the presynaptic side of synaptic junctions.
Information by UniProt
Brain-derived HLMN protein antibody
Western blot - Anti-Piccolo antibody (ab110427)
All lanes : Anti-Piccolo antibody (ab110427) at 1 µg/ml Lane 1 : Mouse brain lysate Lane 2 : Rat brain lysate Lysates/proteins at 10 µg per lane. Predicted band size: 567 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Piccolo antibody (ab110427)
ab110427 at 1/400 staining Piccolo in neuromuscular junction of whole muscle sample from adult mouse by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Secondary used was Alexa Fluor® 488 goat anti-mouse IgG, IgM (H+L). Red staining was an antibody directed against BTX.
has not yet been referenced specifically in any publications.
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