Anti-Piccolo 抗体 [6H9-B6] (ab101654)
Key features and details
- Mouse monoclonal [6H9-B6] to Piccolo
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-Piccolo antibody [6H9-B6]
Piccolo 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [6H9-B6] to Piccolo -
由来種
Mouse -
特異性
No cross reactivity to Bassoon. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Full length protein corresponding to Mouse Piccolo.
Database link: Q9QYX7 -
ポジティブ・コントロール
- WB: Rat and mouse brain tissue lysate. ICC/IF: SK-N-BE cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
6H9-B6 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101654の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 567 kDa.
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|
ICC/IF |
1/100.
|
特記事項 |
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WB
1/1000. Predicted molecular weight: 567 kDa. |
ICC/IF
1/100. |
ターゲット情報
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機能
May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking. -
配列類似性
Contains 2 C2 domains.
Contains 1 PDZ (DHR) domain. -
ドメイン
C2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change. -
細胞内局在
Cell junction > synapse. Concentrated at the presynaptic side of synaptic junctions. - Information by UniProt
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参照データベース
- Entrez Gene: 27445 Human
- Entrez Gene: 26875 Mouse
- Entrez Gene: 56768 Rat
- Omim: 604918 Human
- SwissProt: Q9Y6V0 Human
- SwissProt: Q9QYX7 Mouse
- SwissProt: Q9JKS6 Rat
- Unigene: 12376 Human
see all -
別名
- ACZ antibody
- Aczonin antibody
- Brain-derived HLMN protein antibody
see all
画像
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SK-N-BE cells labeling Piccolo using ab101654 (green) at 1/100 dilution in ICC/IF. Cells were fixed using 4% formaldehyde for 15 minutes at room temperature. Primary antibody was incubated for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 at 1/100 dilution for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).
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All lanes : Anti-Piccolo antibody [6H9-B6] (ab101654) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Predicted band size: 567 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab101654 は 1 報の論文で使用されています。
- Mateos-Hernández L et al. Quantitative proteomics reveals Piccolo as a candidate serological correlate of recovery from Guillain-Barré syndrome. Oncotarget 7:74582-74591 (2016). ELISA ; Human . PubMed: 27776345