The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa.
Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
Expressed in all tissues examined.
Note=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10.
Immunofluorescence of PICALM in Human brain tissue with ab106409 at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence - Anti-PICALM antibody (ab106409)This image is courtesy of Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.
ICC/IF image of PICALM staining on blood smear using ab106409 (1:200). The blood film was fixed in paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106409 was diluted 1200 using and incubated with the cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000)
Western blot - PICALM antibody (ab106409)
All lanes : Anti-PICALM antibody (ab106409) at 1 µg/ml
Lane 1 : EL4 cell lysate Lane 2 : EL4 cell lysate with immunizing peptide