製品の概要

  • 製品名Anti-PICALM antibody
    PICALM 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PICALM
  • 特異性At least three isoforms of PICALM are known to exist; ab106409 will recognize all three.
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A 15 amino acid peptide from near the amino terminus of Human PICALM (NP_009097).

  • ポジティブ・コントロール
    • EL4 cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab106409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 71 kDa.

ターゲット情報

  • 機能Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
  • 組織特異性Expressed in all tissues examined.
  • 関連疾患Note=A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10.
  • 配列類似性Contains 1 ENTH (epsin N-terminal homology) domain.
  • 細胞内局在Membrane > clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle. Colocalized with clathrin in the Golgi area.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CALM antibody
    • Clathrin assembly lymphoid myeloid leukemia antibody
    • Clathrin assembly lymphoid myeloid leukemia protein antibody
    • CLTH antibody
    • LAP antibody
    • Phosphatidylinositol-binding clathrin assembly protein antibody
    • PICAL_HUMAN antibody
    • PICALM antibody
    see all

Anti-PICALM antibody 画像

  • Immunofluorescence of PICALM in Human brain tissue with ab106409 at 20 ug/mL.

  • ICC/IF image of PICALM staining on blood smear using ab106409 (1:200). The blood film was fixed in paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106409 was diluted 1200 using and incubated with the cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000)

  • All lanes : Anti-PICALM antibody (ab106409) at 1 µg/ml

    Lane 1 : EL4 cell lysate
    Lane 2 : EL4 cell lysate with immunizing peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 71 kDa

Anti-PICALM antibody (ab106409) 使用論文

ab106409 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Human Serum (Serum)
Specification Serum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Aug 14 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Human Cell (Blood smear)
Specification Blood smear
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Sep 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"