製品の概要

  • 製品名Anti-PIAS3 antibody [PIA3]
    PIAS3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PIA3] to PIAS3
  • 特異性This antibody recognized PIAS3.
  • アプリケーション適用あり: ELISA, ICC, WBmore details
  • 種交差性
    交差種: Mouse, Chicken, Cow, Dog, Human
  • 免疫原

    Synthetic peptide:

    GGALREGHGGPLPS

    , corresponding to amino acids 591-604 of Human PIAS3

  • ポジティブ・コントロール
    • HepG2 total cell extract.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab59547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
ICC
WB
  • 追加情報ELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 68 and additional band at 44 kDa may be present (predicted molecular weight: 67 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
    • 組織特異性Widely expressed.
    • パスウェイProtein modification; protein sumoylation.
    • 配列類似性Belongs to the PIAS family.
      Contains 1 PINIT domain.
      Contains 1 SAP domain.
      Contains 1 SP-RING-type zinc finger.
    • ドメインThe PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
      The LXXLL motif is a transcriptional coregulator signature.
    • 翻訳後修飾Sumoylated.
    • 細胞内局在Cytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.
    • Information by UniProt
    • 参照データベース
    • 別名
      • E3 SUMO protein ligase PIAS 3 antibody
      • E3 SUMO protein ligase PIAS3 antibody
      • E3 SUMO-protein ligase PIAS3 antibody
      • FLJ14651 antibody
      • OTTHUMP00000015586 antibody
      • OTTHUMP00000015587 antibody
      • PIAS 3 antibody
      • Pias3 antibody
      • PIAS3 protein antibody
      • PIAS3_HUMAN antibody
      • Protein inhibitor of activated STAT 3 antibody
      • Protein inhibitor of activated STAT protein 3 antibody
      • Protein inhibitor of activated STAT3 antibody
      • Zinc finger MIZ type containing 5 antibody
      • ZMIZ 5 antibody
      • ZMIZ5 antibody
      see all

    Anti-PIAS3 antibody [PIA3] (ab59547) 使用論文

    ab59547 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab59547.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"