製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PIAS3
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide, conjugated to KLH, from the C terminal region of Human PIAS3.

  • ポジティブ・コントロール
    • K562 cell lysate; Human Skin and Prostate tissues.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 68 kDa.
IHC-P Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
  • 組織特異性
    Widely expressed.
  • パスウェイ
    Protein modification; protein sumoylation.
  • 配列類似性
    Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • ドメイン
    The PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
    The LXXLL motif is a transcriptional coregulator signature.
  • 翻訳後修飾
    Sumoylated.
  • 細胞内局在
    Cytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 SUMO protein ligase PIAS 3 antibody
    • E3 SUMO protein ligase PIAS3 antibody
    • E3 SUMO-protein ligase PIAS3 antibody
    • FLJ14651 antibody
    • OTTHUMP00000015586 antibody
    • OTTHUMP00000015587 antibody
    • PIAS 3 antibody
    • Pias3 antibody
    • PIAS3 protein antibody
    • PIAS3_HUMAN antibody
    • Protein inhibitor of activated STAT 3 antibody
    • Protein inhibitor of activated STAT protein 3 antibody
    • Protein inhibitor of activated STAT3 antibody
    • Zinc finger MIZ type containing 5 antibody
    • ZMIZ 5 antibody
    • ZMIZ5 antibody
    see all

画像

  • ab119106, at 5 µg/ml, staining PIAS3 in Formalin-fixed, Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • ab119106, at 5 µg/ml, staining PIAS3 in Formalin-fixed, Paraffin-embedded Human Skin tissue by Immunohistochemistry.
  • Anti-PIAS3 antibody (ab119106) at 1 µg/ml + K562 cell lysate

    Predicted band size : 68 kDa

参考文献

ab119106 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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