製品の概要

  • 製品名Anti-PIAS2 antibody
    PIAS2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PIAS2
  • アプリケーション適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 115-131 of Human PIASx.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 63 kDa.
ELISA 1/1000.

ターゲット情報

  • 機能Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'.
  • 組織特異性Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.
  • パスウェイProtein modification; protein sumoylation.
  • 配列類似性Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • 発生段階Isoform 3 expression in adult testis is 14.2-fold stronger than in embryonic testis.
  • ドメインThe LXXLL motif is a transcriptional coregulator signature.
  • 翻訳後修飾Sumoylated.
  • 細胞内局在Nucleus speckle. Nucleus, PML body. Nucleus. Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs) (PubMed:22406621). Colocalizes with SUMO1 in nuclear granules (By similarity).
  • Information by UniProt
  • 参照データベース
  • 別名
    • Androgen receptor interacting protein 3 antibody
    • Androgen receptor-interacting protein 3 antibody
    • ARIP3 antibody
    • DAB2 interacting protein antibody
    • DAB2-interacting protein antibody
    • DIP antibody
    • E3 SUMO protein ligase PIAS2 antibody
    • E3 SUMO-protein ligase PIAS2 antibody
    • MIZ antibody
    • Miz1 antibody
    • Msx interacting zinc finger protein antibody
    • Msx-interacting zinc finger protein antibody
    • PIAS NY protein antibody
    • PIAS-NY protein antibody
    • PIAS2 antibody
    • PIAS2_HUMAN antibody
    • PIASX antibody
    • PIASX-ALPHA antibody
    • PIASX-BETA antibody
    • Protein inhibitor of activated STAT x antibody
    • Protein inhibitor of activated STAT, 2 antibody
    • Protein inhibitor of activated STAT2 antibody
    • SIZ2 antibody
    • Zinc finger, MIZ-type containing 4 antibody
    • ZMIZ4 antibody
    see all

Anti-PIAS2 antibody 画像

  • Western blot analysis of anti-PIASx1/2 Pab (ab4902) in Y79 cell lysate. PIASx1/2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • ab4902 staining PIAS2 in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-PIAS2 antibody (ab4902) 使用論文

ab4902 has not yet been referenced specifically in any publications.

Product Wall

This antibody should detect a band at approximately 63kDa and you advise that the major band you find is at approximately 55 kDa. It is possible that this 55 kDa band could correspond to PIASx as it is not too far off the predicted size. You have tried...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"