リコンビナント
RabMAb

Anti-PIAS1 抗体 [EPR2580(2)] (ab109388)

製品の概要

  • 製品名
    Anti-PIAS1 antibody [EPR2580(2)]
    PIAS1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2580(2)] to PIAS1
  • アプリケーション
    適用あり: WB, IHC-P, ICC, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PIAS1 aa 50-150. The exact sequence is proprietary.

  • ポジティブ・コントロール
    • Human colon tissue. HepG2, Jurkat, 293T, Daudi cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-PIAS1 antibody (Alexa Fluor® 647) [EPR2580(2)] (ab203328)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling.
    • 組織特異性
      Expressed in numerous tissues with highest level in testis.
    • パスウェイ
      Protein modification; protein sumoylation.
    • 配列類似性
      Belongs to the PIAS family.
      Contains 1 PINIT domain.
      Contains 1 SAP domain.
      Contains 1 SP-RING-type zinc finger.
    • ドメイン
      The LXXLL motif is a transcriptional coregulator signature.
      The SP-RING-type domain is required for promoting EKLF sumoylation.
    • 翻訳後修飾
      Sumoylated.
      Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene.
    • 細胞内局在
      Nucleus speckle. Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AR interacting protein antibody
      • DDXBP1 antibody
      • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 antibody
      • DEAD/H box binding protein 1 antibody
      • DEAD/H box-binding protein 1 antibody
      • E3 SUMO-protein ligase PIAS1 antibody
      • GBP antibody
      • Gu binding protein antibody
      • Gu-binding protein antibody
      • GU/RH-II antibody
      • Pias1 antibody
      • PIAS1_HUMAN antibody
      • Protein inhibitor of activated STAT protein 1 antibody
      • Protein inhibitor of activated STAT, 1 antibody
      • RNA helicase II binding protein antibody
      • RNA helicase II-binding protein antibody
      • Zinc finger, MIZ-type containing 3 antibody
      • ZMIZ3 antibody
      see all

    Anti-PIAS1 antibody [EPR2580(2)] 画像

    • Lane 1 : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/500 dilution
      Lane 2 : Anti-GST antibody [EPR4236] (ab111947) at 1/2000 dilution

      Lane 1 : Recombinant Human PIAS1 protein (ab152888)
      Lane 2 : Recombinant Human PIAS1 protein (ab152888)

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 72 kDa
      Observed band size : 98 kDa (why is the actual band size different from the predicted?)

      Exposure time:

      Lane 1: 10 seconds.

      Lane 2: 1 minute.

       

      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-PIAS1 antibody [EPR2580(2)] (ab109388) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : HepG2 cell lysates
      Lane 3 : 392T cell lysates
      Lane 4 : Daudi cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 72 kDa
    • Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/100 ab109388.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-PIAS1 antibody [EPR2580(2)] (ab109388) 使用論文

    This product has been referenced in:
    • Du L  et al. Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal. Nat Commun 7:12326 (2016). IHC-P ; Human . Read more (PubMed: 27465491) »
    • Campia I  et al. An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells. PLoS One 10:e0126159 (2015). WB . Read more (PubMed: 25955018) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 31 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録