Anti-PI4K2A 抗体 - N-terminal (ab71824)

製品の概要

  • 製品名
    Anti-PI4K2A antibody - N-terminal
    PI4K2A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PI4K2A - N-terminal
  • アプリケーション
    適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide from the N-terminal region of human PI4K2A conjugated to KLH

  • ポジティブ・コントロール
    • HL60 cell lysate. Human hepatocarcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

ターゲット情報

  • 関連性
    Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) PI4K2A contributes to the overall PI4 kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells
  • 細胞内局在
    Cytoplasm. Membrane. Note: Found in subdomains of the plasma membrane termed non caveolar membrane rafts.
  • 参照データベース
  • 別名
    • Phosphatidylinositol 4 kinase type 2 alpha antibody
    • Phosphatidylinositol 4 kinase type II alpha antibody
    • PI4KII antibody

画像

  • Anti-PI4K2A antibody - N-terminal (ab71824) at 1/100 dilution + HL60 cell lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • ab71824 at 1/50 dilution, staining PI4K2A in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

参考文献

ab71824 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 mouse fibroblast cell line)
Loading amount
40 µg
Specification
NIH 3T3 mouse fibroblast cell line
Treatment
48 hs-serum deprived
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. GERMAN GIL

Verified customer

投稿 Sep 22 2011

Thank you for your reply and further enquiry. I can confirm that only four nonconsecutive amino acids of the immunogenic sequence for ab71824 PI4K2A antibody are identical to PI4K2B. Although this has not been tested in the laboratory, it is unlike...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (multiple myeloma)
Total protein in input
300 µg
Specification
multiple myeloma
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

投稿 Jan 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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