The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
PI3 Kinase p85 gamma (PIK3R3) binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
It is an heterodimer of a p110 (catalytic) and a p55 (regulatory) subunits. It is expressed at highest levels in brain and testis; at lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.