The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
1/50 - 1/100.
Use a concentration of 5 µg/ml.
機能Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
配列類似性Belongs to the PI3K p85 subunit family. Contains 1 Rho-GAP domain. Contains 2 SH2 domains. Contains 1 SH3 domain.
ドメインThe SH2 2 domain is required for interaction with FBXL2 and PTPN13.
翻訳後修飾Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317). Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.
ICC/IF image of ab62901 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab62901 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab62901 at 1/50 - 1/100 dilution, staining PI 3 Kinase p85 beta in human brain by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue. ab62901 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot - PI 3 Kinase p85 beta antibody (ab62901)
All lanes : Anti-PI 3 Kinase p85 beta antibody (ab62901) at 1/100 dilution
Lane 1 : 293 cell lysates, nontransfected Lane 2 : 293 cell lysates, transiently transfected with the PI 3 Kinase p85 beta gene
Lysates/proteins at 2 µg per lane.
Predicted band size : 82 kDa Observed band size : 82 kDa Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Anti-PI 3 Kinase p85 beta antibody (ab62901) 使用論文
has not yet been referenced specifically in any publications.