Anti-PI 3 Kinase p85 alpha + Gamma 抗体 (ab74136)

製品の概要

  • 製品名Anti-PI 3 Kinase p85 alpha + Gamma antibody
  • 製品の詳細
    Rabbit polyclonal to PI 3 Kinase p85 alpha + Gamma
  • 特異性PI3 kinase p85 alpha and gamma.
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human PI 3 Kinase p85 alpha around the phosphorylation site of tyrosine 468 (R-L-YP-E-E).

  • ポジティブ・コントロール
    • Extracts from COS7 cells treated with H2O2 (100µM, 30mins). HeLa cells. Human breast carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 84 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500.

ターゲット情報

  • 関連性The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
  • 細胞内局在Cytoplasmic
  • 参照データベース
  • 別名
    • GRB1 antibody
    • p55 antibody
    • p55 GAMMA antibody
    • p85 alpha antibody
    • p85 antibody
    • Phosphatidylinositol 3 kinase associated p85 alpha antibody
    • Phosphatidylinositol 3 kinase associated p85 gamma antibody
    • Phosphatidylinositol 3 kinase regulatory 1 antibody
    • Phosphatidylinositol 3 kinase regulatory 3 antibody
    • PI3K antibody
    • PIK3R1 antibody
    • PIK3R3 antibody
    • PtdIns 3 kinase p85 alpha antibody
    • PtdIns 3 kinase p85 gamma antibody
    see all

Anti-PI 3 Kinase p85 alpha + Gamma antibody 画像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab74136, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • Immunofluorescence analysis of HeLa cells using ab74136, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • All lanes : Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells, treated with H2O2 (100µM, 30mins)
    Lane 2 : Extracts from COS7 cells, treated with H2O2 (100µM, 30mins) with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 84 kDa
    Observed band size : 84 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • ab74136 at 1/200 dilution staining PI 3 Kinase p85 alpha + Gamma in mouse embryonic stem cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized in 1:1 methanol, acetone and blocked in 1% serum for 30 minutes at 25°C. The primary antibody was used at 1/100 dilution in Goat block dillution buffer and incubated with sample at 25°C for 1 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG (H+L) was used as secondary at 1/500 dilution.

    See Abreview

Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136) 使用論文

This product has been referenced in:
  • Bhattacharyya S  et al. Amelioration of aspirin induced oxidative impairment and apoptotic cell death by a novel antioxidant protein molecule isolated from the herb Phyllanthus niruri. PLoS One 9:e89026 (2014). Mouse . Read more (PubMed: 24586486) »
  • Tural D  et al. Clinical significance of p95HER2 overexpression, PTEN loss and PI3K expression in p185HER2-positive metastatic breast cancer patients treated with trastuzumab-based therapies. Br J Cancer 110:1968-76 (2014). Human . Read more (PubMed: 24595002) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (undifferentiated human iPS cells)
Specification undifferentiated human iPS cells
Blocking step 5% milk in TBST as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

投稿 Jan 29 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (embryonic stem cells)
Specification embryonic stem cells
Fixative Paraformaldehyde
Permeabilization Yes - 1:1 methanol:acetone
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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Verified customer

投稿 Jan 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"