Anti-PI 3 Kinase p85 alpha 抗体 [EP380Y] (ab40755)


  • 製品名Anti-PI 3 Kinase p85 alpha antibody [EP380Y]
    PI 3 Kinase p85 alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP380Y] to PI 3 Kinase p85 alpha
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to C-terminal (Human)

  • ポジティブ・コントロール
    • A431, MCF7 or Raji cells lysate, HeLa cells
  • 特記事項

    A trial size is available to purchase for this antibody.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab40755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).


  • 機能Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
  • 組織特異性Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
  • 配列類似性Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.
  • ドメインThe SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
  • 翻訳後修飾Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GRB1 antibody
    • p85 alpha antibody
    • p85 antibody
    • P85A_HUMAN antibody
    • Phosphatidylinositol 3 kinase associated p 85 alpha antibody
    • Phosphatidylinositol 3 kinase regulatory 1 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
    • Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
    • Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
    • PI3 kinase p85 subunit alpha antibody
    • PI3-kinase regulatory subunit alpha antibody
    • PI3-kinase subunit p85-alpha antibody
    • PI3K antibody
    • PI3K regulatory subunit alpha antibody
    • Pik3r1 antibody
    • PtdIns 3 kinase p85 alpha antibody
    • PtdIns-3-kinase regulatory subunit alpha antibody
    • PtdIns-3-kinase regulatory subunit p85-alpha antibody
    see all

Anti-PI 3 Kinase p85 alpha antibody [EP380Y] 画像

  • Predicted band size : 85 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PI 3 Kinase p85 alpha knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: THP1 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab40755 observed at 90 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab40755 was shown to recognize PI 3 Kinase p85 alpha when PI 3 Kinase p85 alpha knockout samples were used, along with additional cross-reactive bands. Wild-type and PI 3 Kinase p85 alpha knockout samples were subjected to SDS-PAGE. ab40755 and ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755) at 1/2000 dilution

    Lane 1 : A431 cells
    Lane 2 : MCF-7 cells
    Lane 3 : Raji cells

    Lysates/proteins at 10 µg per lane.

    Predicted band size : 85 kDa
    Observed band size : 85 kDa

Anti-PI 3 Kinase p85 alpha antibody [EP380Y] (ab40755) 使用論文

This product has been referenced in:
  • Yan LX  et al. PIK3R1 targeting by miR-21 suppresses tumor cell migration and invasion by reducing PI3K/AKT signaling and reversing EMT, and predicts clinical outcome of breast cancer. Int J Oncol 48:471-84 (2016). IHC-P ; Human . Read more (PubMed: 26676464) »
  • Hu J  et al. Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling. PLoS One 9:e89352 (2014). WB ; Mouse . Read more (PubMed: 24551245) »

See all 8 Publications for this product

Product Wall

Application Western blot
Loading amount 75 µg
Gel Running Conditions Reduced Denaturing (8)
Sample Mouse Tissue lysate - whole (Brain tissue)
Specification Brain tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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投稿 Nov 20 2014

Thank you for your reply.

I am sorry that the protocol tips did not prove to be successful. As requested I have refunded the cost of this antibody. Your credit note number is ********.

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Thank you for contacting Abcam. I am sorry that you are having problems with ab40755 in IHC-P on human tissue. I have attached the protocol that was used to test this antibody originally in IHC-P and the only suggestion I can make after reading the pro...

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