製品の概要

  • 製品名Anti-PI 3 Kinase Class 3 antibody
    PI 3 Kinase Class 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PI 3 Kinase Class 3
  • アプリケーション適用あり: IHC-P, ICC/IF, ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 39-54 of Human PI3KC 3.

  • ポジティブ・コントロール
    • HeLa cell lysate.
  • 特記事項


    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • 一次抗体 備考Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 101 kDa (predicted molecular weight: 106 kDa).

ターゲット情報

  • 機能Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis.
  • 組織特異性Ubiquitously expressed, with a highest expression in skeletal muscle.
  • 配列類似性Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.
  • 細胞内局在Midbody.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hVps34 antibody
    • MGC61518 antibody
    • Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody
    • Phosphatidylinositol 3 kinase class 3 antibody
    • Phosphatidylinositol 3 kinase p100 subunit antibody
    • Phosphatidylinositol 3-kinase catalytic subunit type 3 antibody
    • Phosphatidylinositol 3-kinase p100 subunit antibody
    • Phosphoinositide 3 kinase class 3 antibody
    • Phosphoinositide-3-kinase class 3 antibody
    • PI3 kinase type 3 antibody
    • PI3-kinase type 3 antibody
    • PI3K type 3 antibody
    • Pik3c3 antibody
    • PK3C3_HUMAN antibody
    • PtdIns 3 kinase type 3 antibody
    • PtdIns-3-kinase type 3 antibody
    • Vps 34 antibody
    • Vps34 antibody
    see all

Anti-PI 3 Kinase Class 3 antibody 画像



  • Predicted band size : 106 kDa


    ab5451 at a 1/100 dilution staining approximately 101kDa band of PI3KC 3 in HeLa cell lysate by Western blot (ECL).
  • ab5451 staining PI 3 Kinase Class 3 in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ICC/IF image of ab5451 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5451, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PI 3 Kinase Class 3 antibody (ab5451) 使用論文

This product has been referenced in:
  • Viana R  et al. Role of AMP-activated protein kinase in autophagy and proteasome function. Biochem Biophys Res Commun 369:964-8 (2008). WB ; Human . Read more (PubMed: 18328803) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"