製品の概要

  • 製品名Anti-PHRF1 antibody
    PHRF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PHRF1
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1400 and 1450 of Human PHRF1 (NP_065952.1).

  • ポジティブ・コントロール
    • HeLa, 293T and NIH3T3 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 179 kDa (predicted molecular weight: 179 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性PHRF1 contains 1 PHD-type zinc finger and 1 RING-type zinc finger. It may interact with POLR2A (via the C-terminal domain).
  • 参照データベース
  • 別名
    • AA673488 antibody
    • CTD binding SR like protein rA9 antibody
    • KIAA1542 antibody
    • PHD and RING finger domain containing protein 1 antibody
    • PHD and ring finger domains 1 antibody
    • PHRF 1 antibody
    • RNF221 antibody
    see all

Anti-PHRF1 antibody 画像

  • All lanes : Anti-PHRF1 antibody (ab85974) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 179 kDa
    Observed band size : 179 kDa


    Exposure time : 10 seconds
  • Detection of PHRF1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85974 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-PHRF1 antibody (ab85974) 使用論文

ab85974 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab85974.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"