PHD and RING finger domain containing protein 1 antibody
PHD and ring finger domains 1 antibody
PHRF 1 antibody
Western blot - PHRF1 antibody (ab85974)
All lanes : Anti-PHRF1 antibody (ab85974) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 179 kDa Observed band size: 179 kDa
Exposure time: 10 seconds
Immunoprecipitation - PHRF1 antibody (ab85974)
Detection of PHRF1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab85974 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.