製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PHPT1
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, from the N-terminal region of human PHPT1.

  • ポジティブ・コントロール
    • PHPT1 transfected 293 cell lysate, human skeletal muscle tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    This antibody was purified through a protein A column, followed by immunogen affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab71326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Detects a band of approximately 14, 60 kDa (predicted molecular weight: 14 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

ターゲット情報

  • 関連性
    PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I. Protein histidine phosphorylation and dephosphorylation in eukaryotes has been sparsely studied compared to protein serine/threonine and tyrosine phosphorylation, although protein histidine phosphorylation is estimated to account for about 6% of total protein phosphorylation in eukaryotes. Ek et al. (2002) detected no activity of PHPT1 towards phosphoserine, phosphothreonine, or phosphotyrosine peptides.
  • 細胞内局在
    Cytoplasmic
  • 参照データベース
  • 別名
    • PHP14 antibody
    • 14 kDa phosphohistidine phosphatase antibody
    • CGI 202 antibody
    • HSPC141 antibody
    • Phosphohistidine phosphatase 1 antibody
    • PHPT 1 antibody
    • Protein janus A homolog antibody
    see all

画像

  • All lanes : Anti-PHPT1 antibody (ab71326) at 1/50 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : PHPT1 transfected 293 cell lysate

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 14 kDa
    Observed band size : 14 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Human skeletal muscle tissue with ab71326 at 1/10 dilution. DAB staining.

参考文献

ab71326 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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