Anti-Phosphotyrosine 抗体 [PY20] (Rhodamine) (ab28346)

製品の概要

  • 製品名Anti-Phosphotyrosine antibody [PY20] (Rhodamine)
    Phosphotyrosine 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PY20] to Phosphotyrosine (Rhodamine)
  • 標識Rhodamine. Ex: 550nm, Em: 570nm
  • 特異性Ab28346 specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.
  • アプリケーション適用あり: ICC, IHC-Fr, WBmore details
  • 種交差性
    交差種: Species independent
  • 免疫原

    Phosphotyrosine coupled to carrier protein.

  • 特記事項

    Ab28346 is especially useful for the detection of these phosphorylated tyrosine proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest. The antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).

    The purified antibody was labeled by reaction with tetramethyl rhodamine (TAMRA) succinimidyl ester in the presence of phenyl phosphate (to protect the antibody binding site). The unreacted TAMRA succinimidyl ester was removed by molecular exclusion chromatography.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use a concentration of 5 - 10 µg/ml.
IHC-Fr Use a concentration of 5 - 10 µg/ml.
WB 1/5000.

ターゲット情報

  • 関連性The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Anti-Phosphotyrosine antibody [PY20] (Rhodamine) (ab28346) 使用論文

ab28346 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"