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Phosphotyrosine coupled to carrier protein.
Ab28346 is especially useful for the detection of these phosphorylated tyrosine proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest. The antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).
The purified antibody was labeled by reaction with tetramethyl rhodamine (TAMRA) succinimidyl ester in the presence of phenyl phosphate (to protect the antibody binding site). The unreacted TAMRA succinimidyl ester was removed by molecular exclusion chromatography.
Our Abpromise guarantee covers the use of ab28346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 5 - 10 µg/ml.|
|IHC-Fr||Use a concentration of 5 - 10 µg/ml.|
ab28346 has not yet been referenced specifically in any publications.