Anti-Phosphotyrosine 抗体 [PY20] (Biotin) (ab16388)

製品の概要

  • 製品名Anti-Phosphotyrosine antibody [PY20] (Biotin)
    Phosphotyrosine 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PY20] to Phosphotyrosine (Biotin)
  • 標識Biotin
  • 特異性Clone PY20 specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.
  • アプリケーション適用あり: WB, ELISA, ICCmore details
  • 種交差性
    交差種: Species independent
  • 免疫原

    Phosphotyrosine coupled to carrier protein

  • 特記事項

    This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).

    The purified antibody is coupled through free primary amino groups to biotin. The binding site of the antibody was protected by the addition of phenyl phosphate prior to coupling.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
ICC
  • 追加情報
    ELISA: 1/5000.
    ICC: 1/500.
    WB: 1/5000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

    Anti-Phosphotyrosine antibody [PY20] (Biotin) (ab16388) 使用論文

    ab16388 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application ELISA
    Sample Human Recombinant protein (YSPTSPS (CTD repeat) peptide)
    Specification YSPTSPS (CTD repeat) peptide
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 22°C
    Type Direct
    Username

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    Verified customer

    投稿 Mar 14 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"