製品の概要

  • 製品名Anti-Phosphotyrosine antibody (HRP)
    Phosphotyrosine 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Phosphotyrosine (HRP)
  • 標識HRP
  • 特異性Recognizes both free phosphotyrosine (pY) and tyrosine-phosphorylated peptide and proteins.
  • アプリケーション適用あり: WB, IP, ELISAmore details
  • 種交差性
    交差種: Species independent
  • 免疫原

    Chemical/ Small Molecule conjugated to KLH.

  • ポジティブ・コントロール
    • Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Anti-Phosphotyrosine antibody (HRP) 画像

  • Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)

Anti-Phosphotyrosine antibody (HRP) (ab9329) 使用論文

This product has been referenced in:
  • McLaughlin NJ  et al. Platelet-Activating Factor-Mediated Endosome Formation Causes Membrane Translocation of p67phox and p40phox That Requires Recruitment and Activation of p38 MAPK, Rab5a, and Phosphatidylinositol 3-Kinase in Human Neutrophils. J Immunol 180:8192-203 (2008). WB . Read more (PubMed: 18523285) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.

We do not have affinity data for this antibody. If you need an HRP-conjugated antibody, I recommend the mouse monoclonal ab16389:

Click here (or use the following: http://www.abcam.com/index.html?datas...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (CD4+ murine splenoyctes)
Loading amount 50000 cells
Specification CD4+ murine splenoyctes
Treatment anti-CD3 and anti-CD28-coated beads
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
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投稿 Nov 27 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"