Anti-Phosphotyrosine 抗体 (ab17302)

製品の概要

  • 製品名Anti-Phosphotyrosine antibody
    Phosphotyrosine 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Phosphotyrosine
  • 特異性Minimal to no crossreactivity with phosphothreonine or phosphoserine containing proteins.
  • アプリケーション適用あり: ELISA, WB, IPmore details
  • 種交差性
    交差種: Species independent
  • 免疫原

    Affinity purified native tyrosine phosphorylated proteins (Human).

  • ポジティブ・コントロール
    • A431 Cell lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab17302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000 - 1/10000.
WB 1/1000 - 1/2000.
IP Use at an assay dependent dilution. Use 2.5 µg pwe IP reaction.

ターゲット情報

  • 関連性The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Anti-Phosphotyrosine antibody 画像

  • All lanes : Anti-Phosphotyrosine antibody (ab17302)

    Lane 1 : Untreated parotid acinar cells
    Lane 2 : Parotid acinar cells with 50µM etoposide for 2 hours
    Lane 3 : Parotid acinar cells with 5mM H2O2 for 30 min

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Donkey anti-Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview

Anti-Phosphotyrosine antibody (ab17302) 使用論文

ab17302 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Parotid acinar cells)
Loading amount 50 µg
Specification Parotid acinar cells
Treatment untreated (UT), + 50uM etoposide for 2 hours (E), + 5mM H2O2 for 30 min (H)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"