特異性Binds specifically and with a high affinity to proteins with phosphorylated tyrosine residues. Binding can be blocked by phosphotyrosine and phenyl phosphate (IC50:10e-6M, ELISA). Does not crossreact with tyrosine, phosphoserine, phosphothreonine, sulphotyrosine (sulfated (23-25) cholecystokinine) or Na-benzoate
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use a concentration of 0.4 - 1 µg/ml.
Use a concentration of 100 µg/ml.
Use a concentration of 5 - 10 µg/ml.
関連性The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.