Anti-Phosphothreonine 抗体 (HRP) (ab9338)

製品の概要

• 製品名
  Anti-Phosphothreonine antibody (HRP)
  Phosphothreonine 一次抗体 製品一覧
• 製品の詳細
  Rabbit polyclonal to Phosphothreonine (HRP)
• 標識
  HRP
• 特異性
  Reacts with free phosphothreonine but does not react with phosphoserine, threonine or phosphotyrosine.
• アプリケーション
  適用あり: WB, IP, ELISAmore details
• 種交差性
  交差種: Species independent
  
• 免疫原

  Chemical/ Small Molecule conjugated to KLH.

• ポジティブ･コントロール
  • Use mouse brain extract for immunoblotting. Use synthetic phosphopeptide (on threonine) for ELISA.

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Store at +4°C.
• バッファー
  Phosphate-Buffered Saline
• Concentration information loading...
• 精製度
  Immunogen affinity purified
• 特記事項（精製）
  Immunoaffinity chromatography with phosphothreonine-agarose.
• ポリ/モノ
  ポリクローナル
• アイソタイプ
  IgG
• 研究分野
  • Signal Transduction
  • Metabolism
  • Amino Acids

  • Signal Transduction
  • Protein Phosphorylation
  • pSer / pThr

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Amino acid metabolism

  • Metabolism
  • Types of disease
  • Cancer

関連製品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Related Products
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  • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
  • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
  • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
  • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
  • 450 nm Stop Solution for TMB Substrate (ab171529)
  • 650 nm Stop Solution for TMB Substrate (ab171531)
  • Immunoassay Blocking Buffer (ab171534)
  • Immunoassay Blocking (BSA Free) (ab171535)

アプリケーション

ターゲット情報

• 関連性
  Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation. Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr. An understanding of transformation by oncogenes and mitogenic processes of growth factors depends on the identification of their substrate and a subsequent determination of how phosphorylation affects their properties. Studies on the role of phosphorylated proteins have been hampered by their low abundance and the problem of distinguishing the various types of phosphorylated proteins. The most common procedure is to label intact cells or small tissue fragments with 32P and subsequently to isolate 32P labeled proteins by conventional biochemical methods. In order to identify the specific amino acids that undergo phosphorylation, additional long and tedious procedures for phosphoamino acid analysis are required. Immunoblotting of cellular proteins with antibodies directed against phosphoamino acids is advantageous as it does not involve 32P labeling, and can therefore be employed to monitor alterations in phosphorylation of specific proteins as they occur in intact organs or the whole animal. Indeed, mono and polyclonal antibodies directed against phosphorylated residues have been generated and found useful as analytical and preparative tools because they enable the rapid identification, quantification and immunoaffinity isolation of phosphorylated cellular proteins.

プロトコール

• Western blot protocols
• Immunoprecipitation protocols