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Our Abpromise guarantee covers the use of ab17464 in the following tested applications.
Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20.
Image courtesy of an anonymous Abreview.The lower blot is to detect total mybpc3. It was probed with a non-abcam anti-mybpc3 antibody.
Image from Gao X et al, PLoS Pathog 5:e1000708 (2009), Fig S3.To determine if NleH could phosphorylate RPS3, we conducted in vitro kinase assays with NleH and RPR3 and analyzed the results by Immunoblotting with ab17464, phospho-Ser/Thr-specific antibody, following separation by SDS-PAGE.
This image is courtesy of an anonymous Abreview