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phospho-serineconjugated to KLH
This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lot is from ascites and is still in stock as of 18th October 2016 - GR197414. Lot numbers higher than GR197414 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Our Abpromise guarantee covers the use of ab6639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Can be blocked with Synthetic Phosphoserine protein (BSA) (ab100812). Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. Stronger blocking agent percentage is recommended (e.g. 10%), in addition to varying loading amounts and shorter incubation time (1-2h RT)|