製品の概要

  • 製品名
    Anti-Phosphoserine antibody [PSR-45]
    Phosphoserine 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PSR-45] to Phosphoserine
  • 特異性
    Monoclonal Anti-Phosphoserine reacts against phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP.
  • アプリケーション
    適用あり: Dot blot, IP, Indirect ELISA, WBmore details
  • 種交差性
    交差種: Species independent
  • 免疫原

    Other Immunogen Type corresponding to Phosphoserine conjugated to keyhole limpet haemocyanin.
    Sequence:

    phospho-serine


    (Peptide available as ab100812)

  • ポジティブ・コントロール
    • WB: rat brain cortex lysate ( a main 50kDa band is seen by detection with biotin-avidin AEC staining) ELISA: phosphoserine conjugated to BSA
  • 特記事項

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot Use at an assay dependent concentration.
IP 1/2000.
Indirect ELISA 1/4000.
WB 1/500 - 1/1000. Can be blocked with Synthetic Phosphoserine protein (BSA) (ab100812). Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. Stronger blocking agent percentage is recommended (e.g. 10%), in addition to varying loading amounts and shorter incubation time (1-2h RT)

ターゲット情報

  • 関連性
    Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • 別名
    • phospho-Ser antibody
    • pS antibody
    • pSER antibody

Anti-Phosphoserine antibody [PSR-45] 画像

  • Anti-Phosphoserine antibody [PSR-45] (ab6639) at 1/200 dilution + Drosophila melanogaster whole tissue lysate 40 ug per lane at 40 µg

    Secondary
    Sheep anti mouse (HRP) at 1/2000 dilution

    Performed under reducing conditions.

    See Abreview

  • ab6639 used in Immunoprecipitation.
    Whole cell lysate prepared from murine mammary cells was loaded at 250µg.
    Cells were treated with 5ng/ml TGF beta for varying time periods.
    Protein A was used for the Immunoprecipitation step.
    ab6639 used at a 1/2000 dilution for 10 hours at 4°C.
    Western blot was performed with an HRP anti-PCBP1 polyclonal antibody, 1/10000 dilution.

    See Abreview

Anti-Phosphoserine antibody [PSR-45] (ab6639) 使用論文

This product has been referenced in:
  • Kusumoto-Matsuo R  et al. Serines 440 and 467 in the Werner syndrome protein are phosphorylated by DNA-PK and affects its dynamics in response to DNA double strand breaks. Aging (Albany NY) 6:70-81 (2014). WB . Read more (PubMed: 24429382) »
  • Cho KI  et al. Differential loss of prolyl isomerase or chaperone activity of Ran-binding protein 2 (Ranbp2) unveils distinct physiological roles of its cyclophilin domain in proteostasis. J Biol Chem 289:4600-25 (2014). WB . Read more (PubMed: 24403063) »

See all 13 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (NSCLC)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
30 µg
Treatment
0-100 nM volasertib for 2 Hrs
Specification
NSCLC
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 11 2017

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Yes, I'd be happy to replace, credit or refund the antibodies. Please just let me know which option is best for you. In case of a r...

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Thank you for your emails and the additional details you sent.

Yes, the use of PBS is a good point. In case of using anti-phospho antibodies this can actually cause high background due to the presence of phosphate in the buffer. I would sugg...

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Thank you for contacting us.

I am sorry to hear that the 2 antibodies have not been working as expected.

Your protocol looks fine in general. However, both antibodies are directed against ANY phospho-site on a serine or threonine o...

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Thank you for your telephone enquiry last week.

I am sorry to confirm that ab6639 Phosphoserine antibody [PSR-45] has only been tested in rat brain tissue bythe originator, and so we have no images for human samples.

There have, h...

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Thank you for contacting us.

To our knowledge this product has not been tested in IHC. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing disc...

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wis...

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Based on the information that you have provided, I do not think that there is any protocol tips that would help resolve the issue, as I think you have tried everything.

Under our Abpromise, which is g...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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