製品の概要

  • 製品名Anti-Phosphoserine antibody (HRP)
    Phosphoserine 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Phosphoserine (HRP)
  • 標識HRP
  • 特異性Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Has slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Species independent
  • 免疫原

    BSA and KLH-phosphoserine conjugates.

  • ポジティブ・コントロール
    • Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding.
ELISA Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
  • 別名
    • phospho-Ser antibody
    • pS antibody
    • pSER antibody

Anti-Phosphoserine antibody (HRP) 画像


  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 5 minutes

Anti-Phosphoserine antibody (HRP) (ab9334) 使用論文

This product has been referenced in:
  • Martínez-Florensa M  et al. Serine residues in the LAT adaptor are essential for TCR-dependent signal transduction. J Leukoc Biol 89:63-73 (2011). WB . Read more (PubMed: 20940326) »
  • Zang ZJ  et al. Identification of PP2A as a novel interactor and regulator of TRIP-Br1. Cell Signal 21:34-42 (2009). WB . Read more (PubMed: 18940248) »

See all 7 Publications for this product

Product Wall



Es gibt eigentlich kein besonderes Protokoll für diesen Antikörper. Die einzigen Abweichungen von unserem Etablierungsprotokoll sind der Vermerk bezüglich des Blockmittels ("To block use 3%BSA with 0.1% gelatin (do not use milk). We recommen...

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Vielen Dank für Ihren Anruf.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456und ...

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Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogens erhalten wir alle nötigen Informationen über Ihre Proben un...

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Thank you very much for following up with this customer. I am sorry that the results were not improve with the suggestions.

As requested, I have added a new free of charge unit of ab9334 of a different batch to your order xx

I hope t...

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Thank you for your inquiry.

I am sorry to hear that you are experiencing difficulty with this antibody. Like we said on the phone, please try diluting the primary antibody more to 1:10,000 for 1 -2 hrs at RT, increasing the blocking to 1% BS...

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Thank you for taking time to liaise with the customer and to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provi...

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The phosphoserine antibodies were tested positive for the following phosphopeptides: RREILSRRPpSYR RRALpSLR TPRpSP

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"