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Our Abpromise guarantee covers the use of ab17465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/8000 - 1/16000.|
|WB||1/500 - 1/1000.
(colorimetric). Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead.
|ICC/IF||Use a concentration of 1 µg/ml.|