Rabbit polyclonal to Phospholipase D2 (phospho Y169)
ab78907 detects endogenous levels of Phospholipase D2 only when phosphorylated at tyrosine 169.
Synthetic phosphopeptide derived from human Phospholipase D2 around the phosphorylation site of tyrosine 169 (E-N-Y
IHC-P: human brain tissue
WB: extracts from Jurkat cells treated with TNF (20 ng/ml, 30 min)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab78907 was purified by affinity chromatography using an epitope specific phosphopeptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 106 kDa.
1/50 - 1/100.
May have a role in signal-induced cytoskeletal regulation and/or endocytosis.
Belongs to the phospholipase D family.
Contains 1 PH domain. Contains 2 PLD phosphodiesterase domains. Contains 1 PX (phox homology) domain.
Information by UniProt
Choline phosphatase 2 antibody
EC 22.214.171.124 antibody
hPLD 2 antibody
Western blot - Anti-Phospholipase D2 (phospho Y169) antibody (ab78907)
All lanes :
Anti-Phospholipase D2 (phospho Y169) antibody (ab78907) at 1/500 dilution
Lane 1 :
extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min)
Lane 2 :
extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min) with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size:
Observed band size:
94 kDa (
why is the actual band size different from the predicted?
Additional bands at:
140 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase D2 (phospho Y169) antibody (ab78907)
Immunohistochemical analysis of Phospholipase D2 in paraffin embedded human brain tissue using ab78907, at 1/50 dilution, in the presence (right image) and absence (left image) of the immunizing peptide.
has not yet been referenced specifically in any publications.
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