The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100-2000. Predicted molecular weight: 129 kDa. Due to the low expression levels of this protein, it is suggested that the protein be immunoprecipitated before Western blotting.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.
Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
Belongs to the phospholipase D family. Contains 1 PH domain. Contains 2 PLD phosphodiesterase domains. Contains 1 PX (phox homology) domain.