The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/250. Detects a band of approximately 150 kDa (predicted molecular weight: 149 kDa).
Is unsuitable for Flow Cyt, ICC/IF, IHC-P or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ. Ubiquitinated by CBLB in activated T-cells.
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
Western blot - Anti-Phospholipase C gamma 1 antibody [EP2522Y] (ab68147)
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: Phospholipase C gamma 1 knockout HAP1 cell lysate (20 µg) Lane 3: HepG2 cell lysate (20 µg) Lane 4: Mouse brain tissue lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab68147 observed at 160 kDa. Red - loading control, ab8245 , observed at 37 kDa.
ab68147 was shown to recognize Phospholipase C gamma 1 when Phospholipase C gamma 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1 knockout samples were subjected to SDS-PAGE. ab68147 at a dilution of 1/00 and ab8245 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Phospholipase C gamma 1 antibody [EP2522Y] (ab68147)
Anti-Phospholipase C gamma 1 antibody [EP2522Y] (ab68147) at 1/500 dilution + Jurkat cell lysate at 10 µg
Secondary HRP-conjugated Gaot anti-rabbit at 1/2000 dilution