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RabMAb

Anti-Phospholipase C gamma 1 抗体 [EP2522Y] (ab68147)

製品の概要

  • 製品名
    Anti-Phospholipase C gamma 1 antibody [EP2522Y]
    Phospholipase C gamma 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2522Y] to Phospholipase C gamma 1
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminus of human Phospholipase C gamma 1.

  • ポジティブ・コントロール
    • Jurkat cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: 1/100 - 1/250. Detects a band of approximately 150 kDa (predicted molecular weight: 149 kDa).

    Is unsuitable for Flow Cyt, ICC/IF, IHC-P or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
    • 配列類似性
      Contains 1 C2 domain.
      Contains 1 EF-hand domain.
      Contains 2 PH domains.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • ドメイン
      The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
    • 翻訳後修飾
      The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
      Ubiquitinated by CBLB in activated T-cells.
    • 細胞内局在
      Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
      • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
      • Inositoltrisphosphohydrolase antibody
      • Monophosphatidylinositol phosphodiesterase antibody
      • NCKAP3 antibody
      • Phosphatidylinositol phospholipase C antibody
      • Phosphoinositidase C antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-1 antibody
      • Phospholipase C 148 antibody
      • Phospholipase C gamma 1 antibody
      • Phospholipase C-gamma-1 antibody
      • Phospholipase C-II antibody
      • PLC gamma 1 antibody
      • PLC II antibody
      • PLC-148 antibody
      • PLC-gamma-1 antibody
      • PLC-II antibody
      • PLC1 antibody
      • PLC148 antibody
      • Plcg1 antibody
      • PLCG1_HUMAN antibody
      • PLCgamma1 antibody
      see all

    画像



    • Predicted band size : 149 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Phospholipase C gamma 1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HepG2 cell lysate (20 µg)
      Lane 4: Mouse brain tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab68147 observed at 160 kDa. Red - loading control, ab8245 , observed at 37 kDa.

      ab68147 was shown to recognize Phospholipase C gamma 1 when Phospholipase C gamma 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1 knockout samples were subjected to SDS-PAGE. ab68147 at a dilution of 1/00 and ab8245 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Anti-Phospholipase C gamma 1 antibody [EP2522Y] (ab68147) at 1/500 dilution + Jurkat cell lysate at 10 µg

      Secondary
      HRP-conjugated Gaot anti-rabbit at 1/2000 dilution

      Predicted band size : 149 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    ab68147 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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