Anti-Phospholipase C gamma 1 抗体 [EP2522Y] (ab68147)


  • 製品名Anti-Phospholipase C gamma 1 antibody [EP2522Y]
    Phospholipase C gamma 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2522Y] to Phospholipase C gamma 1
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminus of human Phospholipase C gamma 1.

  • ポジティブ・コントロール
    • Jurkat cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.




Our Abpromise guarantee covers the use of ab68147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
  • 追加情報WB: 1/100 - 1/250. Detects a band of approximately 150 kDa (predicted molecular weight: 149 kDa).

    Is unsuitable for Flow Cyt, ICC/IF, IHC-P or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
    • 配列類似性Contains 1 C2 domain.
      Contains 1 EF-hand domain.
      Contains 2 PH domains.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
      Contains 2 SH2 domains.
      Contains 1 SH3 domain.
    • ドメインThe SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1.
    • 翻訳後修飾The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
      Ubiquitinated by CBLB in activated T-cells.
    • 細胞内局在Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
      • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
      • Inositoltrisphosphohydrolase antibody
      • Monophosphatidylinositol phosphodiesterase antibody
      • NCKAP3 antibody
      • Phosphatidylinositol phospholipase C antibody
      • Phosphoinositidase C antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-gamma-1 antibody
      • Phospholipase C 148 antibody
      • Phospholipase C gamma 1 antibody
      • Phospholipase C-gamma-1 antibody
      • Phospholipase C-II antibody
      • PLC gamma 1 antibody
      • PLC II antibody
      • PLC-148 antibody
      • PLC-gamma-1 antibody
      • PLC-II antibody
      • PLC1 antibody
      • PLC148 antibody
      • Plcg1 antibody
      • PLCG1_HUMAN antibody
      • PLCgamma1 antibody
      see all

    Anti-Phospholipase C gamma 1 antibody [EP2522Y] 画像

    • Predicted band size : 149 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Phospholipase C gamma 1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HepG2 cell lysate (20 µg)
      Lane 4: Mouse brain tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab68147 observed at 160 kDa. Red - loading control, ab8245 , observed at 37 kDa.

      ab68147 was shown to recognize Phospholipase C gamma 1 when Phospholipase C gamma 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1 knockout samples were subjected to SDS-PAGE. ab68147 at a dilution of 1/00 and ab8245 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Anti-Phospholipase C gamma 1 antibody [EP2522Y] (ab68147) at 1/500 dilution + Jurkat cell lysate at 10 µg

      HRP-conjugated Gaot anti-rabbit at 1/2000 dilution

      Predicted band size : 149 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    Anti-Phospholipase C gamma 1 antibody [EP2522Y] (ab68147) 使用論文

    ab68147 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab68147.
    Please use the links above to contact us or submit feedback about this product.