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Synthetic peptide within Human Phospholipase C gamma 1. The exact sequence is proprietary.
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab76155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).|
|ICC||1/250 - 1/500.
ab76155 can lose reactivity with Phospholipase C gamma 1 when it is phosphoryalted on Tyr783, only in ICC. This was observed when Jurkat cells were treated with 1mM pervanadate for 30mins. This was not rescued by Lamda phosphatase treatment.
|Flow Cyt||Use at an assay dependent concentration.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Phospholipase C gamma 1 knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: Mouse brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76155 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76155 was shown to specifically react with Phospholipase C gamma 1 when Phospholipase C gamma 1 knockout samples were used. Wild-type and Phospholipase C gamma 1 knockout samples were subjected to SDS-PAGE. ab76155 at a dilution of 1/5000 and ab8245 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry analysis of Jurkat ( Human acute T cell leukemia) labeling Phospholipase C gamma 1 with purified ab76155 at 1/200 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"