Anti-Phospholipase C gamma 1/PLC-gamma-1 抗体 (ab52200)
Key features and details
- Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody
Phospholipase C gamma 1/PLC-gamma-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, African green monkey
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1.
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ポジティブ・コントロール
- Human breast carcinoma tissue (IHC-P).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52200の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa). |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
配列類似性
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
ドメイン
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
翻訳後修飾
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
細胞内局在
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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参照データベース
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- SwissProt: P10686 Rat
- Unigene: 268177 Human
see all -
別名
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
画像
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Paraffin-embedded human breast carcinoma tissue stained for Phospholipase C gamma 1/PLC-gamma-1, using ab52200 (1/100). The right hand panel represents a negative control where ab52200 was pre-incubated with the immunizing (blocking) peptide.
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All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody (ab52200) at 1/500 dilution
Lane 1 : Extracts from COS7 cells treated
with EGF (200ng/ml, 30min).
Lane 2 : Extracts from COS7 cells treated
with EGF (200ng/ml, 30min). For this panel, ab52200 was pre-icubated with the immunizing (blocking) peptide.
Predicted band size: 149 kDa
Observed band size: 149 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab52200 は 1 報の論文で使用されています。
- Filhol O et al. CK2β Is a Gatekeeper of Focal Adhesions Regulating Cell Spreading. Front Mol Biosci 9:900947 (2022). PubMed: 35847979