Anti-Phospholipase C epsilon 1 抗体 (ab121476)

製品の概要

• 製品名
  Anti-Phospholipase C epsilon 1 antibody
  Phospholipase C epsilon 1 一次抗体 製品一覧
• 製品の詳細
  Rabbit polyclonal to Phospholipase C epsilon 1
• アプリケーション
  適用あり: IHC-Pmore details
• 種交差性
  交差種: Human
  
• 免疫原

  antigen, corresponding to amino acids 161-295 of Human Phospholipase C epsilon 1, Isoform 1.

• ポジティブ･コントロール
  • Human colon tissue.

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• バッファー
  pH: 7.20
  Preservative: 0.02% Sodium azide
  Constituents: 59% PBS, 40% Glycerol
• Concentration information loading...
• 精製度
  Immunogen affinity purified
• ポリ/モノ
  ポリクローナル
• アイソタイプ
  IgG
• 研究分野
  • Signal Transduction
  • Signaling Pathway
  • Lipid Signaling
  • PLC

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

関連製品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

アプリケーション

ターゲット情報

• 機能
  The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation.
• 組織特異性
  Widely expressed. Isoform 1 is broadly expressed and only absent in peripheral blood leukocytes. Isoform 2 is specifically expressed in placenta, lung and spleen.
• 関連疾患
  Defects in PLCE1 are the cause of nephrotic syndrome type 3 (NPHS3) [MIM:610725]; also known as early-onset nephrotic syndrome type 3. Nephrotic syndrome, a malfunction of the kidney glomerular filter, leads to proteinuria, hypoalbuminemia, edema. End-stage kidney disease is observed in steroid-resistant nephrotic syndrome.
• 配列類似性
  Contains 1 C2 domain.
  Contains 1 PI-PLC X-box domain.
  Contains 1 PI-PLC Y-box domain.
  Contains 2 Ras-associating domains.
  Contains 1 Ras-GEF domain.
• ドメイン
  The Ras-associating domain 1 is degenerated and may not bind HRAS. The Ras-associating domain 2 mediates interaction with GTP-bound HRAS, RAP1A, RAP2A and RAP2B and recruitment of HRAS to the cell membrane.
  The Ras-GEF domain has a GEF activity towards HRAS and RAP1A. Mediates activation of the mitogen-activated protein kinase pathway.
• 細胞内局在
  Cytoplasm > cytosol. Cell membrane. Golgi apparatus membrane. Recruited to plasma membrane by activated HRAS and RAP2. Recruited to perinuclear membrane by activated RAP1A. Isoform 1 and isoform 2 associates with Golgi membranes.

• Target information above from: UniProt accession Q9P212 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 51196 Human
  • Omim: 608414 Human
  • SwissProt: Q9P212 Human
  • Unigene: 655033 Human
• 別名
  • 1-phosphatidylinositol-4 antibody
  • 5-bisphosphate phosphodiesterase epsilon-1 antibody
  • Pancreas-enriched phospholipase C antibody

  • Phosphoinositide phospholipase C-epsilon-1 antibody
  • Phospholipase C-epsilon-1 antibody
  • PLC-epsilon-1 antibody
  • Plce1 antibody
  • PLCE1_HUMAN antibody

  see all

プロトコール

• Immunohistochemistry protocols