Anti-Phospholipase C beta 3/PLCB3 (phospho S537) 抗体 [EPR1498Y] (ab51247)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1498Y] to Phospholipase C beta 3/PLCB3 (phospho S537)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y]
Phospholipase C beta 3/PLCB3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1498Y] to Phospholipase C beta 3/PLCB3 (phospho S537) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HeLa whole cell lysate (ab150035).
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1498Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51247の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
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特記事項 |
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WB
1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa). |
ターゲット情報
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機能
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. -
配列類似性
Contains 1 C2 domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5331 Human
- Omim: 600230 Human
- SwissProt: Q01970 Human
- Unigene: 523761 Human
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別名
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase beta-3 antibody
see all
画像
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All lanes : Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y] (ab51247) at 1/2000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 139 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab51247 は論文での使用が確認できていません。