Anti-Phospholipase C beta 3 (phospho S537) 抗体 (ab73998)

製品の概要

  • 製品名Anti-Phospholipase C beta 3 (phospho S537) antibody
    Phospholipase C beta 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Phospholipase C beta 3 (phospho S537)
  • 特異性ab73998 detects endogenous levels of Phospholipase C beta 3 only when phosphorylated at serine 537.
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    synthesized phosphopeptide derived from human Phospholipase C beta 3 around the phosphorylation site of serine 537 (Q-K-SP-L-G).

  • ポジティブ・コントロール
    • IHC-P: Human brain tissue WB: Extracts from Jurkat cells treated with UV (15mins) IF/ICC: HCT116 cell line.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody against non phosphopeptide was removed by chromatography using nonbphosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab73998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 139 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
  • 配列類似性Contains 1 C2 domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase beta-3 antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-beta-3 antibody
    • Phospholipase C beta 3 antibody
    • Phospholipase C beta 3 (phosphatidylinositol specific) antibody
    • Phospholipase C-beta-3 antibody
    • PLC beta 3 antibody
    • PLC-beta-3 antibody
    • Plcb3 antibody
    • PLCB3_HUMAN antibody
    see all

Anti-Phospholipase C beta 3 (phospho S537) antibody 画像

  • ICC/IF image of ab73998 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73998, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • All lanes : Anti-Phospholipase C beta 3 (phospho S537) antibody (ab73998) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells, treated with UV (15mins)
    Lane 2 : Extracts from Jurkat cells, treated with UV (15mins) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 139 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab73998. Left: untreated sample. Right: sample treated with immunising phosphopeptide.

Anti-Phospholipase C beta 3 (phospho S537) antibody (ab73998) 使用論文

ab73998 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Sample Human Cell (Hela cells)
Specification Hela cells
Permeabilization Yes - 0,1% Saponin / PBS
Fixative Methanol
Username

Abcam user community

Verified customer

投稿 Oct 09 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer (pH6.0)
Sample Mouse Tissue sections (brain tissue)
Specification brain tissue
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 09 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gel)
Sample Human Cell lysate - whole cell (Hela cells)
Specification Hela cells
Treatment see additional notes
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 29 2014

Thank you for confirming this information and for your help and cooperation with this case.
As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.
Read More

Thank you for your message and for your patience.

I am sorry that the suggestions did not improve her results on this occation.As her order is within the Abpromise, I would like to offer her a replacement or a credit note.

Thank y...

Read More

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

Read More

Thank you for contacting us with your question. I have issued the discount code below. Please let me know if you have any further questions or if there is anything else that we can do for you.

DISCOUNT CODE: ***
Expiration date: August 3,...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"