Anti-Phospholipase C beta 3 (phospho S537) 抗体 (ab73998)


  • 製品名Anti-Phospholipase C beta 3 (phospho S537) antibody
    Phospholipase C beta 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Phospholipase C beta 3 (phospho S537)
  • 特異性ab73998 detects endogenous levels of Phospholipase C beta 3 only when phosphorylated at serine 537.
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    synthesized phosphopeptide derived from human Phospholipase C beta 3 around the phosphorylation site of serine 537 (Q-K-SP-L-G).

  • ポジティブ・コントロール
    • IHC-P: Human brain tissue WB: Extracts from Jurkat cells treated with UV (15mins) IF/ICC: HCT116 cell line.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody against non phosphopeptide was removed by chromatography using nonbphosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab73998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 139 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.


  • 機能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
  • 配列類似性Contains 1 C2 domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase beta-3 antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-beta-3 antibody
    • Phospholipase C beta 3 antibody
    • Phospholipase C beta 3 (phosphatidylinositol specific) antibody
    • Phospholipase C-beta-3 antibody
    • PLC beta 3 antibody
    • PLC-beta-3 antibody
    • Plcb3 antibody
    • PLCB3_HUMAN antibody
    see all

Anti-Phospholipase C beta 3 (phospho S537) antibody 画像

  • ICC/IF image of ab73998 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73998, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • All lanes : Anti-Phospholipase C beta 3 (phospho S537) antibody (ab73998) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells, treated with UV (15mins)
    Lane 2 : Extracts from Jurkat cells, treated with UV (15mins) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 139 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab73998. Left: untreated sample. Right: sample treated with immunising phosphopeptide.

Anti-Phospholipase C beta 3 (phospho S537) antibody (ab73998) 使用論文

ab73998 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Sample Human Cell (Hela cells)
Specification Hela cells
Permeabilization Yes - 0,1% Saponin / PBS
Fixative Methanol

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Verified customer

投稿 Oct 09 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer (pH6.0)
Sample Mouse Tissue sections (brain tissue)
Specification brain tissue
Permeabilization No
Fixative Paraformaldehyde

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Verified customer

投稿 Oct 09 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gel)
Sample Human Cell lysate - whole cell (Hela cells)
Specification Hela cells
Treatment see additional notes
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

投稿 Aug 29 2014

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