Anti-Phospholipase C beta 3 抗体 [EPR5951] (ab124735)

製品の概要

  • 製品名Anti-Phospholipase C beta 3 antibody [EPR5951]
    Phospholipase C beta 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5951] to Phospholipase C beta 3
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human Phospholipase C beta 3.

  • ポジティブ・コントロール
    • HCT 116, 293T, and A431 cell lysates; Human breast and colon tissues.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
    • 配列類似性Contains 1 C2 domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
      • 1-phosphatidylinositol-4 antibody
      • 5-bisphosphate phosphodiesterase beta-3 antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-beta-3 antibody
      • Phospholipase C beta 3 antibody
      • Phospholipase C beta 3 (phosphatidylinositol specific) antibody
      • Phospholipase C-beta-3 antibody
      • PLC beta 3 antibody
      • PLC-beta-3 antibody
      • Plcb3 antibody
      • PLCB3_HUMAN antibody
      see all

    Anti-Phospholipase C beta 3 antibody [EPR5951] 画像

    • All lanes : Anti-Phospholipase C beta 3 antibody [EPR5951] (ab124735) at 1/1000 dilution

      Lane 1 : HCT 116 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 139 kDa
    • ab124735 at 1/100 dilution staining Phospholipase C beta 3 in paraffin-embedded Human breast tissue by Immunohistochemistry.
    • ab124735 at 1/100 dilution staining Phospholipase C beta 3 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Anti-Phospholipase C beta 3 antibody [EPR5951] (ab124735) 使用論文

    ab124735 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab124735.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"