製品の概要

  • 製品名
    Anti-Phospholipase C beta 3 antibody
    Phospholipase C beta 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Phospholipase C beta 3
  • 特異性
    This antibody detects endogenous levels of total Phospholipase C beta 3 protein.
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    A synthetic non-phosphopeptide designed around the phosphorylation site of serine 1105, of human Phospholipase C beta 3.

  • ポジティブ・コントロール
    • Human brain tissue (IHC).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 137 kDa (predicted molecular weight: 137 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
  • 配列類似性
    Contains 1 C2 domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase beta-3 antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-beta-3 antibody
    • Phospholipase C beta 3 antibody
    • Phospholipase C beta 3 (phosphatidylinositol specific) antibody
    • Phospholipase C-beta-3 antibody
    • PLC beta 3 antibody
    • PLC-beta-3 antibody
    • Plcb3 antibody
    • PLCB3_HUMAN antibody
    see all

画像

  • Paraffin-embedded human brain tissue stained for phospholipase C beta 3, using ab52199 (1/100). The right hand panel represents a negative control in which ab52199 has been pre-incubated with the immunizing (blocking) peptide.
  • All lanes : Anti-Phospholipase C beta 3 antibody (ab52199) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells treated with PMA (125ng/ml, 30min).
    Lane 2 : Extracts from HeLa cells treated with PMA (125ng/ml, 30min). For this panel, ab52199 was pre-incubated with the immunizing (blocking) peptide.


    Predicted band size : 137 kDa
    Observed band size : 137 kDa

参考文献

This product has been referenced in:
  • Béziau DM  et al. Expression of phosphoinositide-specific phospholipase C isoforms in native endothelial cells. PLoS One 10:e0123769 (2015). WB, IHC-P . Read more (PubMed: 25875657) »
  • Feng J  et al. Thromboxane-induced contractile response of human coronary arterioles is diminished after cardioplegic arrest. Ann Thorac Surg 92:829-36 (2011). WB ; Human . Read more (PubMed: 21871266) »

See all 2 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gel)
Sample
Human Cell lysate - whole cell (Hela cells)
Specification
Hela cells
Treatment
see additional notes
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Sep 01 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer (pH6.0)
Sample
Human Tissue sections (lung)
Specification
lung
Permeabilization
No
Fixative
Paraformaldehyde
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投稿 Aug 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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