Anti-Phospholipase C beta 1 抗体 [K92-3 and K32-3] (ab77743)

製品の概要

  • 製品名Anti-Phospholipase C beta 1 antibody [K92-3 and K32-3]
    Phospholipase C beta 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [K92-3 and K32-3] to Phospholipase C beta 1
  • 特異性ab77743 is specific to Phospholipase C beta 1. It also recognizes the 100 kDa and 41 kDa fragments of Phospholipase C beta 1.1
  • アプリケーション適用あり: IHC-P, IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Full length native protein (purified) corresponding to Cow Phospholipase C beta 1.
    Database link: P10894

  • ポジティブ・コントロール
    • Bovine brain membrane preparation. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml.
IP Use at 5-1 µg/mg of lysate.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 138 kDa. PubMed: 24501170

ターゲット情報

  • 機能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
  • 関連疾患Epileptic encephalopathy, early infantile, 12
  • 配列類似性Contains 1 C2 domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • 細胞内局在Nucleus membrane. Cytoplasm. Colocalizes with the adrenergic receptors, ADREN1A and ADREN1B, at the nuclear membrane of cardiac myocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase beta 1 antibody
    • 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate antibody
    • 1-phosphatidylinositol 4 antibody
    • 5-bisphosphate phosphodiesterase beta-1 antibody
    • EIEE12 antibody
    • Inositoltrisphosphohydrolase antibody
    • Monophosphatidylinositol phosphodiesterase antibody
    • Phosphb antibody
    • Phosphoinositidase C antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-beta 1 antibody
    • Phosphoinositide phospholipase C-beta-1 antibody
    • Phospholipase C beta 1 (phosphoinositide-specific) antibody
    • Phospholipase C I antibody
    • Phospholipase C-beta-1 antibody
    • Phospholipase C-I antibody
    • PI PLC antibody
    • PLC 1 antibody
    • PLC beta 1 antibody
    • PLC I antibody
    • PLC-154 antibody
    • PLC-beta-1 antibody
    • PLC-I antibody
    • PLC154 antibody
    • Plcb antibody
    • Plcb1 antibody
    • Plcb1 protein antibody
    • PLCB1_HUMAN antibody
    • PLCbeta1 antibody
    • Triphosphoinositide phosphodiesterase antibody
    see all

Anti-Phospholipase C beta 1 antibody [K92-3 and K32-3] 画像

  • IP/WB
    5 µg of ab77743 was used to immunoprecipitate Phospholipase C beta 1 from 1 mg of a bovine brain membrane preparation. The immunoprecipitate was resolved by electrophoresis, transferred to nitrocellulose and probed with ab77743 (5 µg/ml). Proteins were visualized using a goat anti mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
    Arrows indicate Phospholipase C beta 1 (150 kDa), heavy (H) and light (L) IgG chains.
  • IHC image of Phospholipase C beta 1 staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77743, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Phospholipase C beta 1 antibody [K92-3 and K32-3] (ab77743) 使用論文

This product has been referenced in:
  • Wright MF  et al. Mechanisms of intracellular calcium homeostasis in developing and mature bovine corpora lutea. Biol Reprod 90:55 (2014). WB ; Cow . Read more (PubMed: 24501170) »

See 1 Publication for this product

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Thank you for confirming these details and for your cooperation. I can confirm that : - the blocking peptide for ab5804 is ab5839 - the immunogen of ab103278 is aa 1155-1184 of the isoform A (SwissProt: Q9NQ66-1) Because we carry over 70,000 ...

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Thank you for contacting us and for sending images of the results obtained with ab5804 and ab77743. 1)  ab5804 : I looked at the image of the blot and I am positive that the band observed above the 250kDa marker is ITPR1. As explained in my previou...

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