製品の概要

  • 製品名
    Anti-Phospholipase A2 X antibody
    Phospholipase A2 X 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Phospholipase A2 X
  • 特異性
    This antibody is specific for Phospholipase A2 X.
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant human Phospholipase A2 X. A 15.5 kDa protein containing 123 amino acid residues of the human Phospholipase A2 X and 16 additional amino acid residues, HisTag. Source of immunogen, E.coli

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: 0.1M Sodium chloride, 0.05M PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Immunoaffinity chromatography on a column with immobilized recombinant human Phospholipase A2 X.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab47111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.

ターゲット情報

  • 機能
    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine.
  • 組織特異性
    Found in spleen, thymus, peripheral blood leukocytes, pancreas, lung, and colon.
  • 配列類似性
    Belongs to the phospholipase A2 family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Group 10 secretory phospholipase A2 antibody
    • Group X secretory phospholipase A2 antibody
    • GX sPLA2 antibody
    • GXPLA2 antibody
    • GXSPLA2 antibody
    • PA2GX_HUMAN antibody
    • Phosphatidylcholine 2 acylhydrolase GX antibody
    • Phosphatidylcholine 2-acylhydrolase 10 antibody
    • Phospholipase A2 group X antibody
    • PLA2G10 antibody
    • SPLA2 antibody
    • sPLA2-X antibody
    • sPLA2X antibody
    see all

画像

  • All lanes : Anti-Phospholipase A2 X antibody (ab47111) at 1 µg/ml

    Lane 1 : Whole cell extracts from RAW264.7 control cells
    Lane 2 : Whole cell extracts from RAW264.7 cells overexpressing Phospholipase A2 X

    Lysates/proteins at 20 µg per lane.

    Secondary
    Horseradish peroxidase-conjugated secondary antibody

    Predicted band size : 17 kDa
    Observed band size : 17 kDa

    Image from Curfs DM et al, J Biol Chem. 2008 Aug 1;283(31):21640-8. Epub 2008 May 28, Fig 1.DOI 10.1074/jbc.M710584200

プロトコール

参考文献

This product has been referenced in:
  • Curfs DM  et al. Macrophage Secretory Phospholipase A2 Group X Enhances Anti-inflammatory Responses, Promotes Lipid Accumulation, and Contributes to Aberrant Lung Pathology. J Biol Chem 283:21640-8 (2008). WB ; Human . Read more (PubMed: 18511424) »

See 1 Publication for this product

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