The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50. PubMed: 22240557
Use a concentration of 2 µg/ml. PubMed: 22240557
Use at an assay dependent dilution.
Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa.
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2-linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined.
Mosher AA et al. Regulation of sPLA2-IID in Human Decidua: Insights Into the Complexity of the Prostaglandin Pathway in Labor. Reprod SciN/A:N/A (2014).
Read more (PubMed: 24440994) »
Li K et al. Secretory Phospholipase A2 Group IID Is Involved in Progesterone-Induced Acrosomal Exocytosis of Human Spermatozoa. J Androl33:975-83 (2012).
WB, ICC/IF, Functional Studies
Read more (PubMed: 22240557) »